论文部分内容阅读
目的：探讨 Ｓ Ｖ４０ 早期基因编码产物大 Ｔ 抗原（ Ｔａｇ） 表达及与抑癌蛋白ｐ Ｒｂ 的相互作用在人脑肿瘤发生发展中的意义。方法：采用免疫共沉淀及 Ｗｅｓｔｅｒｎ 印迹法检测４３ 例人脑肿瘤组织及５ 例正常人脑组织中 Ｔａｇ 的表达，并对１５ 例 Ｔａｇ 阳性瘤组织检测 Ｔａｇ － ｐ Ｒｂ 复合物的存在。结果： Ｔａｇ 在５ 例室管膜瘤及２ 例脉络丛乳头状瘤中全部表达，垂体腺瘤（５／６） 、星形胶质细胞瘤（７／１０） 、脑膜瘤（４／６） 、多形性胶质母细胞瘤（２／５） 及髓母细胞瘤（１／５） 均有 Ｔａｇ 的表达； ３ 例少枝胶质细胞瘤、１ 例松果体瘤及５ 例正常人脑组织无 Ｔａｇ 表达。１５ 例 Ｔａｇ 阳性瘤组织中均发现 Ｔａｇ 与ｐ Ｒｂ 形成特异性复合物。结论：在人脑肿瘤组织中 Ｔａｇ 广泛表达， Ｔａｇ 可与ｐ Ｒｂ 形成特异性复合物， Ｔａｇｐ Ｒｂ特异性复合物的形成导致ｐ Ｒｂ 失活，可能是人脑肿瘤发生发展的一个重要机理。
OBJECTIVE: To investigate the significance of the interaction between the expression of large T-tag (T-tag) and the tumor suppressor protein pRb in the early stage of SV40 gene in the development of human brain tumors. METHODS: The expression of Tag in 43 cases of human brain tumor tissues and 5 cases of normal human brain tissues was detected by co-immunoprecipitation and Western blot, and the presence of the Tag-p Rb complex was detected in 15 cases of Tag-positive tumor tissues. Results: Tag was expressed in 5 cases of ependymoma and 2 cases of choroid plexus papilloma. Pituitary adenoma (5/6), astrocytoma (7/10), and meningioma (4/6) , pleomorphic glioblastoma (2/5) and medulloblastoma (1/5) all had Tag expression; 3 cases of oligodendroglioma, 1 case of pineal tumor and 5 cases of normal Brain tissue without Tag expression. A specific complex of Tag and pRb was found in 15 cases of Tag-positive tumor tissues. Conclusion: Tag is widely expressed in human brain tumor tissues, and Tag can form a specific complex with p Rb. The formation of a Tagp Rb specific complex leads to the inactivation of p Rb, which may be an important mechanism for the development of human brain tumors. .