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Objective: Previous studies have shown that glioma cells induced the migration of nerve stem cells (NSCs) in vivo. This study was exploring whether this situation could happen in vitro. Methods: Supernatant from C6 glioma cell lines or astrocytes cultured in serum-free medium growing in logarithmic phase were separately placed in lower chambers and NSCs were placed in the upper chambers of Trans-well culture system. After 36 h co-incubation, these NSCs spheres occurred on the middle membrane were counted. Results: Results demonstrated that the supernatant from C6 glioma cell culture, not from the astrocytes, enhanced the migration of NSCs (P<0.01). Conclusion: Some components in C6 glioma cell culture can attract the migration of NSCs.
Objective: Previous studies have shown that glioma cells induced the migration of nerve stem cells (NSCs) in vivo. This study was exploring whether this situation could happen in vitro. Methods: Supernatant from C6 glioma cell lines or astrocytes cultured in serum-free medium growing in logarithmic phase were separately placed in lower chambers and NSCs were placed in the upper chamber of Trans-well culture system. After 36 h co-incubation, these NSCs spheres occurred on the middle membrane were counted. Results: Results that the supernatant from C6 glioma cell culture, not from the astrocytes, enhanced the migration of NSCs (P <0.01). Conclusion: Some components in C6 glioma cell culture can attract the migration of NSCs.