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目的:探讨靶向抑制Rad50对头颈部鳞癌细胞系放疗敏感性的影响。方法:用siRNA技术建立并筛选Rad50靶向抑制的Tca8113和OSCC-15稳转细胞系,联合应用单次小剂量放疗(2 Gy)处理细胞,Western blot检测Rad50的表达变化;通过Comet assay检测细胞核DNA的双链断裂情况,细胞克隆形成实验检测细胞存活分数的变化;Telomere FISH检测细胞端粒长度的变化。结果:与单次小剂量放疗(2 Gy)处理组相比较,Rad50靶向抑制联合放疗处理的Tca8113和OSCC-15稳转细胞系,Rad50蛋白表达显著降低,细胞核DNA的双链断裂损伤明显加重,细胞增殖速率明显减慢,细胞核内染色体端粒的长度均明显变短。结论:靶向抑制Rad50可以显著增强Tca8113和OSCC-15细胞的放疗敏感性。
OBJECTIVE: To investigate the effect of targeted radiotherapy on the radiosensitivity of head and neck squamous cell carcinoma cell lines. Methods: We established and screened Rad50-targeted Tca8113 and OSCC-15 stable cell lines with siRNA. The cells were treated with a single low-dose radiotherapy (2 Gy) and the expression of Rad50 was detected by Western blot. The nuclei were detected by Comet assay DNA double strand breaks, cell clone formation assay to detect changes in cell viability; Telomere FISH detection of cell telomere length changes. Results: Compared with single-dose low-dose radiotherapy (2 Gy), Rad50-targeted radiotherapy-induced Tca8113 and OSCC-15 stable cell lines showed a significant decrease in Rad50 protein expression and double-strand DNA damage , The rate of cell proliferation was significantly slowed down, the telomere length in the nucleus was significantly shorter. Conclusion: Targeted inhibition of Rad50 can significantly enhance the radiosensitivity of Tca8113 and OSCC-15 cells.