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目的了解东莞市病毒性腹泻的病原学分布情况。方法收集2010年7月至2012年9月东莞市东华医院感染性腹泻患者的粪便样品,采用ELISA方法检测轮状病毒,采用Realtime RT-PCR方法检测诺如病毒,采用RT-PCR方法检测星状病毒,采用Realtime PCR方法检测腺病毒。结果检测粪便样品共224份,诺如病毒、轮状病毒、腺病毒、星状病毒检测阳性率分别为17.9%、11.6%、4.5%,0.4%,总检测阳性率为31.3%。对其中22份轮状病毒阳性样品进行血清分型,结果G血清型中,G1型10株,G2型1株,G3型8株,G9型3株。P血清型中,P6型1株,P8型12株,9株未能分型。40株诺如病毒全部为GⅡ型。1株星状病毒经测序分析为1型。结论东莞市腹泻病毒主要感染3岁以下的婴幼儿,诺如病毒和轮状病毒为主要病原体,轮状病毒的分布呈多样性,以G1型为主。
Objective To understand the etiological distribution of viral diarrhea in Dongguan City. Methods Stool samples from patients with infectious diarrhea in Donghua Hospital of Dongguan City from July 2010 to September 2012 were collected. Rotavirus was detected by ELISA and Norovirus by Realtime RT-PCR. RT-PCR was used to detect stool Like virus, adenovirus detection Realtime PCR method. Results A total of 224 stool samples were detected. The positive rates of norovirus, rotavirus, adenovirus and astrovirus were 17.9%, 11.6%, 4.5% and 0.4%, respectively. The positive rate of total detection was 31.3%. Twenty-two rotavirus positive samples were serotyped. As a result, among the G serotypes, there were 10 strains of G1 type, 1 strain of G2 type, 8 strains of G3 type and 3 strains of G9 type. P serotypes, P6 type 1, P8 type 12 strains, 9 strains failed to type. 40 strains of Norovirus are all G Ⅱ type. One astrovirus was sequenced as type 1. Conclusions Dongguan City, the main diarrhea virus in infants under 3 years of age, norovirus and rotavirus as the main pathogens, rotavirus showed a diversity of distribution, with G1-based.