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对几十个对称与不对称取代的均四嗪衍生物做了红外光谱，并对其中具有代表意 义的样品做了１３Ｃ核磁共振谱，发现不同取代基对苯环及均四嗪环电子云分布都有 不同的影响．表现在红外光谱和１３Ｃ核磁共振都具有相一致的规律性。
Dozens of symmetrical and asymmetric substituted are tetrazine derivatives made by infrared spectroscopy, and representatives of the sample which made 13C nuclear magnetic resonance spectrum and found that different substituents on the benzene ring and the average four ring electron cloud distribution Have different effects. Performance in the infrared spectroscopy and 13C NMR have a consistent regularity.