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目的探讨埃克替尼对鼻咽高分化鳞癌细胞系CNE-1的放射增敏作用。方法细胞培养鼻咽癌CNE-1,分为空白对照组、埃克替尼组(0.094、0.188、0.376μmol·L-1)、照射组(照射剂量分别为0、2、4、8 Gy)和实验组(各浓度埃克替尼+各剂量照射组),利用多靶单击数学模型拟合放射剂量-细胞存活曲线,MTT法观察药物对体外培养的CNE-1细胞凋亡的影响,荧光染色法检测凋亡细胞,流式细胞术分析药物对CNE-1细胞周期的影响,高倍光镜下观察细胞形态。结果埃克替尼作用后,0.094、0.188、0.376μmol·L-1的放射增敏比分别为1.095、1.433、1.639,随药物浓度增加而变大;通过流式细胞仪检测发现,埃克替尼和放射治疗具有协同作用,可使CNE-1细胞周期发生改变,凋亡指数增加,0.376μmol·L-1埃克替尼+照射8Gy的凋亡指数达(50.82±6.54)%。结论埃克替尼对鼻咽高分化鳞癌细胞系CNE-1有明显的放射增敏效应。
Objective To investigate the radiosensitization of imatinib on nasopharyngeal carcinoma cell line CNE-1. Methods CNE-1 cells were cultured in vitro and divided into blank control group, imatinib group (0.094,0.188,0.376μmol·L-1) and irradiation group (irradiation dose were 0, 2, 4 and 8 Gy respectively) And the experimental group (Icotinib at various concentrations + irradiation dose groups), the radiation dose-cell survival curves were fitted by using a multi-target single-click mathematical model and the effects of the drugs on the apoptosis of CNE-1 cells cultured in vitro were observed by MTT method. Apoptotic cells were detected by fluorescent staining. The effects of drugs on the cell cycle of CNE-1 cells were analyzed by flow cytometry. The cell morphology was observed under high light microscope. Results The radiosensitivities of 0.094, 0.188 and 0.376 μmol·L-1 after Icotinib treatment were 1.095, 1.433 and 1.669, respectively, which increased with the increase of the drug concentration. The results of flow cytometry showed that, Neodymium and radiotherapy had a synergistic effect, which could change the cell cycle of CNE-1 and increase the apoptosis index. The apoptosis index of 0.376μmol·L-1 icitin + irradiated 8Gy was (50.82 ± 6.54)%. Conclusion Icitinib has significant radiosensitizing effect on nasopharyngeal carcinoma cell line CNE-1.