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目的:观察复方丹参方所含成分丹参提取物、三七总皂苷、冰片与穿山龙总皂苷配伍对大鼠心肌缺血再灌注损伤的保护作用。方法:SD大鼠70只,随机分为10组:假手术组、模型组、丹参提取物300 mg.kg-1、三七总皂苷100 mg.kg-1、穿山龙总皂苷100 mg.kg-1、配伍(丹参提取物+三七总皂苷+冰片+穿山龙总皂苷)3个剂量组(300+100+10+50),(300+100+10+100),(300+100+10+200)mg.kg-1、复方丹参片组300 mg.kg-1、阳性药组(通心络胶囊)300 mg.kg-1,各组ig给药,连续7 d,末次给药1 h后,结扎冠状动脉左前降支复制心肌缺血再灌注损伤模型,试剂盒测定血清超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)、肌酸激酶同工酶(CK-MB)活性,TTC染色法测定心肌梗死面积,TUNEL染色法检测细胞凋亡。结果:配伍(300+100+10+100)mg.kg-1与模型组比较,可显著增强SOD的活性(P<0.01),降低大鼠心肌中LDH和CK-MB的活性(P<0.01);与各成分和原方相比,心肌梗死范围为20.78%,明显低于丹参提取物34.04%(P<0.05)、三七总皂苷34.62%(P<0.05)、穿山龙总皂苷36.19%(P<0.05)、复方丹参片26.47%;细胞凋亡指数为16.53%,明显低于丹参提取物25.08%(P<0.05)、三七总皂苷22.12%(P<0.05)、穿山龙总皂苷24.45%(P<0.05)、复方丹参片18.14%。结论:复方丹参方与穿山龙总皂苷配伍后可对心肌缺血再灌注损伤起到保护作用,且效果优于各组分及复方丹参方。此种配伍的方式可以作为研究中药新复方的一种方法。
OBJECTIVE: To observe the protective effect of compatibility of Radix Salviae miltiorrhizae, Radix Notoginseng, Borneol and Radix Saponins on myocardial ischemia-reperfusion injury in rats. Methods: Seventy SD rats were randomly divided into 10 groups: sham operation group, model group, Salvia miltiorrhiza extract 300 mg.kg-1, Panax notoginseng saponin 100 mg.kg-1, (300 + 100 + 10 + 100), (300 + 100 + 10 + 100), (300 + 100 + 10 + 50) 200 mg · kg-1, 300 mg · kg-1 for compound Danshen Tablet and 300 mg · kg-1 for Tongxinluo Capsule. The rats in each group were treated with ig for 7 days continuously for 1 h After the left anterior descending artery of the coronary artery was ligated, the model of myocardial ischemia-reperfusion injury was established. The activity of serum superoxide dismutase (SOD), lactate dehydrogenase (LDH) and creatine kinase MB (CK-MB) The area of myocardial infarction was determined by TTC staining and the apoptosis was detected by TUNEL staining. Results: Compared with the model group, the activity of SOD and the activity of LDH and CK-MB in myocardium of rats were significantly increased (P <0.01) by the combination of (300 + 100 + 10 + 100) mg.kg- ). The range of myocardial infarction was 20.78%, which was significantly lower than that of Salvia miltiorrhiza extract 34.04% (P <0.05), Panax notoginseng saponins 34.62% (P <0.05) P <0.05). The apoptosis index of compound Salvia miltiorrhiza Bunge was 26.47% and 16.53%, which was significantly lower than that of Salvia miltiorrhiza Bunge (25.08%, P <0.05) (P <0.05), compound Danshen tablets 18.14%. Conclusion: The combination of compound Salvia miltiorrhiza and Radix Saponins could protect myocardial ischemia-reperfusion injury and its effect is better than that of each component and Salvia miltiorrhiza. Such a combination of ways can be used as a new method of research TCM compound.