许多病原菌的确定要通过分离和培养工作,并且在适当的条件下进行接种试验以肯定它的致病性。此外,为了进一步研究病原菌的形态、生活史、生理和生态,分离获得真菌的纯培养是必要的。人工分离所得病原菌,其形态特征与自然发生的多数是一致的,即在病斑上所发生的是分生孢子堆或分生孢子器,在人工培养时也同样发生。本试验对沙枣褐斑病菌的分离培养出现了不同的情 Many pathogenic bacteria are identified through the work of isolation and culture, and vaccination tests are conducted under appropriate conditions to confirm its pathogenicity. In addition, in order to further study the morphology, life history, physiology and ecology of pathogenic bacteria, it is necessary to obtain pure culture of fungi separately. Artificially isolated from the pathogen, its morphological characteristics and nature of the majority of the same, that is, lesions occurred in the conidia or conidia, artificial cultivation also occurs. In this experiment, different conditions appeared in the isolation and culture of Carassius auratus