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利用台农4号凤梨植株生长点,经一定水平激素的无菌培养、诱导、分化形成丛生芽,在适宜的培养条件下培养25～30d,即可增殖1代,平均1个芽1代可增殖3～3-5个芽。采用机械损伤分生组织,激化分生组织细胞分化,可提高芽条增殖系数。有效芽条经转移生根培养可获得完整植株,移栽室外苗床成活率可达98%以上。
The growth of pineapple branches of TaiNong No.4 was induced by aseptic culture of certain level of hormones to induce the formation of clustered buds and cultured under appropriate culture conditions for 25-30 days to proliferate for one generation with an average of one bud per generation Proliferation of 3 to 3-5 buds. Adopting mechanical injury meristems and intensifying meristem cells differentiation can increase bud proliferation coefficient. The effective buds can be obtained by transferring the rooted culture complete plant survival rate of transplanting seedbed up to 98%.