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从新疆染病的西瓜分离到一种线状病毒,该病毒可经汁液传播侵染属于6个科的17种草本植物;染病的西瓜、哈密瓜和西葫芦引起植株生长阻滞、叶片皱缩、褪绿、泡斑、黄花叶以至坏死等系统侵染症状;该病毒可经桃蚜以非持久性方式传播;该病毒的病毒粒子为长约750nm的线形病毒粒子,在染病组织中形成风轮状内含体,证明该病毒属于马铃薯Y病毒组;染病组织粗汁液和提纯病毒制备物在SDS双向免疫扩散试验中与小西葫芦黄花叶病毒抗血清发生强烈的免疫沉淀反应;以差速离心和蔗糖密度梯度离心提纯了该病毒,其A260/A280为1.22,以提纯病毒制备了经ELISA试验效价为1:109的抗血清.建议将此病毒称为小西葫芦黄花叶病毒新疆株(Zucchiniyellowmosaicvirus-Xinjiang,ZYMV—XJ).
A linear virus was isolated from the diseased watermelon in Xinjiang, which spreads through the juice to 17 herbaceous plants belonging to 6 families; the diseased watermelon, cantaloupe and zucchini cause plant growth retardation, folds, chlorosis , Spot blotch, yellow mosaic and necrosis system infection symptoms; the virus can be spread in a non-persistent manner Myzus persicae virus particles of the virus is about 750nm linear virus particles in the infected tissue to form a wind wheel Containing body, the virus belongs to the potato Y virus group; infected tissue crude juice and purified virus preparations in the SDS two-way immunodiffusion test with the small squash yellow mosaic virus antiserum occurred strong immunoprecipitation; differential centrifugation and sucrose density The virus was purified by gradient centrifugation and its A260 / A280 was 1.22. An antiserum titer of 1: 109 was prepared by ELISA. It is suggested that this virus be called Zucchiniyellow mosaic virus-Xinjiang (ZYMV-XJ).