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目的探讨核因子(NF)-κBp65反义寡核苷酸(ASODN)对实验性结肠炎裸鼠(BALB/c)结肠炎症、外周血T细胞及其T亚群的影响。方法采用2,4,6-三硝基苯磺酸(TNBS)灌肠造模,并将造模型成功的40只BALB/c小鼠均分为模型对照组(UC组)和NF-κBp65反义寡核苷酸组(ASODN组)、错义寡核苷酸组(MSODN组);另设正常对照组(NC组)。于造模后24h对ASODN组、MSODN组小鼠分别进行ASODN、MSODN灌肠治疗。灌肠后第7天处死小鼠,收集结肠组织进行病理评分,收集外周血于流式细胞仪进行T细胞及其亚群测定。结果ASODN组小鼠的结肠炎症评分较UC组和MSODN组明显改善[(5.26±0.88)vs.(10.32±1.25)和(10.40±1.28)](P<0.05),但仍高于NC组的(1.95±0.50)(P<0.05)。(2)ASODN组小鼠T淋巴细胞、Th细胞百分比值、Th/Ts比值较UC组、MSODN组明显升高(P<0.01),Ts细胞百分比则有降低(P<0.05)。结论TNBS能损伤小鼠的细胞免疫功能,NF-κBp65 ASODN能修复并建立新的免疫平衡,改善结肠炎症。
Objective To investigate the effects of nuclear factor (NF) -κBp65 antisense oligonucleotide (ASODN) on colonic inflammation, peripheral blood T cells and T subsets in experimental colitis nude mice (BALB / c). Methods Twenty-four BALB / c mice were divided into model control group (UC group) and NF-κBp65 antisense model by using 2,4,6-trinitrobenzene sulfonic acid (TNBS) Oligonucleotide group (ASODN group), missense oligonucleotide group (MSODN group); another normal control group (NC group). ASODN group and MSODN group were treated with ASODN and MSODN enema 24h after modeling. Mice were sacrificed on the 7th day after enema, colonic tissues were harvested for pathological evaluation, and peripheral blood was collected for flow cytometry for T cells and their subpopulations. Results Compared with UC and MSODN groups, the scores of colonic inflammation in ASODN group were significantly improved [(5.26 ± 0.88) vs. (10.32 ± 1.25) and (10.40 ± 1.28)] (P <0.05), but still higher than those in NC group (1.95 ± 0.50) (P <0.05). (2) The percentage of T lymphocytes, Th cells and Th / Ts in ASODN group were significantly higher than those in UC and MSODN groups (P <0.01), while the percentage of Ts cells was decreased (P <0.05). Conclusion TNBS can damage cellular immune function in mice. NF-κB p65 ASODN can repair and establish new immune balance and improve colonic inflammation.