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在酵母和人类中,依赖于ATP的Lon蛋白酶起着降解线粒体内非正常蛋白质和维持线粒体DNA的稳定等重要作用。本研究应用RT-PCR和RACE技术,克隆了小麦Lon蛋白酶家族的一个基因,命名为TaLon1。TaLon1和籼稻Lon1、玉米Lon1的相似性分别为94%和92%。表达分析表明,TaLon1在小麦根、叶和花药中均表现组成型表达,表明该基因在小麦的生长发育过程中很重要,起着类似管家基因的作用。在菜豆上已经间接证明Lon蛋白酶与细胞质雄性不育有一定的相关性。但在本研究中,TaLon1的表达在小麦K型细胞质雄性不育系和其他育性正常的品系之间没有差别。与大肠杆菌和酵母中的Lon基因不同,TaLon1不能被90 min的42℃热激所诱导。另外,250 mmol/L NaCl处理24 h后,该基因的表达量下降。
In yeast and humans, ATP-dependent Lon protease plays an important role in the degradation of non-normal mitochondrial proteins and in the maintenance of mitochondrial DNA stabilization. In this study, a gene of wheat Lon protease family was cloned by RT-PCR and RACE technology and named as TaLon1. Similarities of TaLon1 to indica Lon1 and maize Lon1 were 94% and 92%, respectively. Expression analysis showed that TaLon1 expressed constitutively in wheat roots, leaves and anthers, indicating that this gene plays an important role in the growth and development of wheat and plays a role of a housekeeping gene. On cabbage it has been indirectly demonstrated that Lon protease has some correlation with cytoplasmic male sterility. However, in this study, TaLon1 expression did not differ between wheat K-type cytoplasmic male sterile lines and other fertile lines. Unlike Lon genes in E. coli and yeast, TaLon1 can not be induced by heat shock at 42 ° C for 90 min. In addition, 250 mmol / L NaCl treatment for 24 h, the gene expression decreased.