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目的研究人工蛹虫草与谷胱甘肽(GSH)组方对酒精性肝损伤的保护作用。方法将小鼠随机分为空白对照组、模型对照组、蛹虫草与GSH低、中、高剂量组(0.200、0.400和1.200 g/kg),灌胃给药,1次/d,连续30 d。实验末期采用50%乙醇一次灌胃,造成小鼠急性肝损伤模型。测定血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平,制作肝组织病理切片,测定肝组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)、三酰甘油(TG)含量。结果 0.400和1.200 g/kg剂量组ALT和AST活性、MDA和TG含量以及肝脏脂肪变性评分,明显低于模型对照组(P<0.05或P<0.01);0.200、0.400和1.200 g/kg剂量组SOD活性,1.200 g/kg剂量组GSH含量明显高于模型对照组(P<0.01)。结论人工蛹虫草与GSH组方对乙醇所致急性肝损伤具有明显的保护作用。
Objective To study the protective effects of Cordyceps militaris and glutathione (GSH) on alcoholic liver injury. Methods Mice were randomly divided into blank control group, model control group, Cordyceps militaris and GSH low, medium and high dose groups (0.200, 0.400 and 1.200 g / kg) . Experimental end of a 50% ethanol once gavage, resulting in acute liver injury model in mice. Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured to make histopathological sections of liver tissue. The levels of superoxide dismutase (SOD), malondialdehyde (MDA), reduced glutathione Peptide (GSH), triglyceride (TG) content. Results The ALT and AST activities, MDA and TG contents, and hepatic steatosis scores in the 0.400 and 1.200 g / kg groups were significantly lower than those in the model control group (P <0.05 or P <0.01); 0.200, 0.400 and 1.200 g / kg dose groups SOD activity, GSH content in 1.200 g / kg dose group was significantly higher than that in model control group (P <0.01). Conclusion The artificial Cordyceps Militaris and GSH prescriptions have significant protective effect on ethanol-induced acute liver injury.