因为氯霉素分子中有酰胺结构,所以它的水溶液制剂很不稳定,易于水解,如直接用分光光度法或重氮化滴定法,不能区别氯霉素和分解产物,因而不能反映氯霉素的真实含量。笔者根据氯霉素在乙酸乙酯中的溶解度比较大的性质,将氯霉素眼药水经盐酸酸化,乙酸乙酯提取后,用紫外分光光度 Because chloramphenicol molecules have amide structure, so its aqueous solution preparation is very unstable, easy to hydrolyze, such as direct spectrophotometry or diazotization titration, can not distinguish between chloramphenicol and decomposition products, which can not reflect the chloramphenicol The real content. Based on the chloramphenicol in ethyl acetate solubility of the larger nature of the chloramphenicol eye drops acidified by hydrochloric acid, ethyl acetate extraction, UV spectrophotometry