论文部分内容阅读
液相色谱以液体方式进样,特别适合于高分子、高沸点和热不稳定物质的分离和分析,补充了气相色谱的不足。液相色谱检定器的作用,是经色谱柱分离后的各组分在经过检测元件时根据浓度的不同转换成电讯号,用记录仪记录出谱图,以进行定性或定量分析。目前,常用的液体色谱检定器有紫外检定器、示差折光检定器、萤光检定器、吸附热检定器和介电常数检定器等,但使用得最广泛的是紫外检定器。它具有灵敏度高,不受温度、压力等外界因素影响的优点。通常的紫外检定器的结构是相当复杂的。它具有精密的光路系统,需要精度高的机械加工和光学加工,自制很不容易。作为紫外范围的光电接收元件,国产的只有 GD-
Liquid-phase injection of liquid chromatography, especially suitable for the separation and analysis of high molecular weight, high boiling point and heat-labile substances, complementing the deficiencies of gas chromatography. The role of LC detector is separated by the column after each component in the detection of components according to the concentration of different converted into electrical signals recorded with a recorder spectrogram for qualitative or quantitative analysis. At present, the commonly used liquid chromatographic detector UV detector, refractive index detector, fluorescence detector, adsorption calorimeter and dielectric constant detector, but the most widely used UV detector. It has a high sensitivity, free from temperature, pressure and other external factors. The structure of a typical UV detector is rather complex. It has a sophisticated optical system, requires high precision machining and optical processing, self-made is not easy. As the UV range of the photoelectric receiver components, made only GD-