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目的探讨缺氧诱导因子-1α(H IF-1α)、金属基质蛋白酶-2(MMP-2)在子宫内膜癌中的表达及其临床意义。方法2006年8月至2009年9月在中国医科大学附属盛京医院应用免疫组化SP法检测52例子宫内膜腺癌、23例子宫内膜不典型增生、18例正常子宫内膜组织中H IF-1α及MMP-2的表达情况。结果H IF-1α、MMP-2在子宫内膜癌组织中的阳性表达率(73.08%,71.15%)明显高于正常子宫内膜组织(22.22%,16.67%)及不典型增生子宫内膜组织(39.13%,34.78%),差异有统计学意义(P<0.05)。随着子宫内膜腺癌临床分期、病理组织学分级的提高,H IF-1α及MMP-2的表达也随之增强;二者在子宫内膜癌有淋巴结转移组中的表达高于无淋巴结转移组,差异有统计学意义(P<0.05)。子宫内膜组织中H IF-1α、MMP-2蛋白的表达呈正相关(rs=0.543,P<0.01)。结论H IF-1α、MMP-2在正常子宫内膜、子宫内膜不典型增生及子宫内膜癌组织中的表达依次增高,提示H IF-1α及MMP-2在子宫内膜癌发生发展中起重要作用,可将其视为子宫内膜癌潜在侵袭性的肿瘤标志物。
Objective To investigate the expression of HIF-1α and MMP-2 in endometrial carcinoma and its clinical significance. Methods From August 2006 to September 2009, 52 cases of endometrial adenocarcinoma, 23 cases of endometrial dysplasia and 18 cases of normal endometrium were examined by immunohistochemical SP method in Shengjing Hospital Affiliated to China Medical University. H IF-1α and MMP-2 expression. Results The positive expression rate of HIF-1α and MMP-2 in endometrial carcinoma was significantly higher than that in normal endometrium (73.2%, 71.6%) and atypical hyperplasia (39.13%, 34.78%), the difference was statistically significant (P <0.05). With the clinical stage and pathological grade of endometrial adenocarcinoma, the expression of HIF-1α and MMP-2 also increased. The expression of HIF-1α and MMP-2 in endometrial carcinoma with lymph node metastasis was higher than that without lymph node metastasis Metastasis group, the difference was statistically significant (P <0.05). The expression of HIF-1α and MMP-2 in endometrium was positively correlated (rs = 0.543, P <0.01). Conclusions The expression of HIF-1α and MMP-2 in normal endometrium, endometrial dysplasia and endometrial carcinoma sequentially increases, suggesting that HIF-1α and MMP-2 may play an important role in the development of endometrial carcinoma Plays an important role as a potential invasive tumor marker of endometrial cancer.