【摘 要】
：
采用大容量二氧化碳超临界流体萃取设备对裂殖壶菌OUC168菌粉进行油脂萃取,并以四种油料作物黑芝麻、大豆、花生、核桃进行比较,通过气相色谱技术检测了萃取物中的脂肪酸组成
【机 构】
：
中国海洋大学海洋生物遗传育种教育部重点实验室,山东青岛,266003
论文部分内容阅读
采用大容量二氧化碳超临界流体萃取设备对裂殖壶菌OUC168菌粉进行油脂萃取,并以四种油料作物黑芝麻、大豆、花生、核桃进行比较,通过气相色谱技术检测了萃取物中的脂肪酸组成及含量,并比较和分析了不同萃取条件下超临界流体对不同脂肪酸的萃取效果.结果 表明,四种油料作物中的脂肪酸均以16-18C为主,裂殖壶菌中含有丰富的长链脂肪酸DHA(22C:6,44.34％),是非常好的不饱和脂肪酸来源.二氧化碳超临界流体萃取技术可以有效萃取四种油料作物中的脂肪酸,萃取率均在75％以上.对于裂殖壶菌中脂肪酸的萃取,在萃取温度30-40℃,萃取压力30MPa,分离温度35-55℃条件下,长链脂肪酸的萃取率总体低于短链脂肪酸;同种脂肪酸在萃取温度为30℃时的萃取率高于40℃时的萃取率.在萃取温度30℃,萃取压力30MPa,分离温度55℃,分离压力8～10MPa,CO2流速14L/h的条件下,二氧化碳超临界流体萃取裂殖壶菌总脂肪酸的萃取率为56.03％,DHA萃取率为22.16％.
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