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目的观察1,25(OH)_2D_3对大鼠CD4~+CD25~+调节性T细胞、IL-10以及大鼠肾移植后存活的影响。方法以SD大鼠为供体,Wister大鼠为受体,建立大鼠肾移植模型。受体随机分为4组：(1)对照组(组Ⅰ)；(2)CsA组(组Ⅱ)：3mg·kg~(-1)·d~(-1)腹腔注射治疗组,术后连续给药13d；(3)1,25(OH)_2D_3组(组Ⅲ)：1μg·kg~(-1)·d~(-1)腹腔注射治疗组,术后连续给药13d；(4) CsA+1,25(OH)_2D_3组(组Ⅳ)：按VD3组和CsA组用药剂量给药。观察移植后4组大鼠存活时间,并于术前4d,术后6、16d检测血清肌酐、IL-10以及术后6d脾脏Treg含量。结果组Ⅲ、组Ⅳ大鼠移植存活时间分别为(13．3±3．2)d和(24．8±2．6)d,较对照组(8．7±1．8d)明显延长(P＜0．01)；组Ⅲ、组Ⅳ移植后6d Cr水平分别为(62．7±7．5)mmol／L、(77．1±9．4)mmol／L,明显低于对照组(424．3±61．2)mmol／L(P＜0．01)。给予1．25(OH)_2D_3后大鼠IL-10水平(268．1±24．3)pg／ml以及脾脏CD4~+CD25~+ T细胞的比率(18．51±3．73)％,较时照组[分别为(50．1±3．0)pg／ml和(8．95±2．01)％]明显增高(P＜0．01)。结论1．25 (OH)_2D_3能显著促进体内IL-10的产生和CD4~+CD25~+T细胞的增殖,适量1,25(OH)_2D_3与CsA联合应用可以明显改善肾功能,延长移植肾的存活时间。
Objective To investigate the effect of 1,25 (OH) _2 D_3 on the survival of CD4 ~ + CD25 ~ + regulatory T cells, IL-10 and rat kidney after renal transplantation. Methods SD rats were used as donors and Wister rats as recipients to establish rat kidney transplantation model. The recipients were randomly divided into 4 groups: (1) control group (group Ⅰ); (2) CsA group (group Ⅱ): intraperitoneal injection of 3 mg · kg -1 · d -1, (3) 1,25 (OH) _2D_3 group (group Ⅲ): 1μg · kg -1 (-1) · d -1 intraperitoneal injection group, continuous administration for 13 days; (4) ) CsA + 1,25 (OH) _2D_3 group (group Ⅳ): according to VD3 group and CsA group dose. The survival time of the four groups of rats after transplantation was observed. The levels of serum creatinine, IL-10 and the content of Treg in the spleen at 6 days after operation were detected at 4 days and 6 and 16 days after operation. Results The survival time of group Ⅲ and group Ⅳ was (13.3 ± 3.2) days and (24.8 ± 2.6) days respectively, which was significantly longer than that of control group (8.7 ± 1.8 days) (P <0.01). The levels of Cr at 6th day after transplantation in group Ⅲ and group Ⅳ were (62.7 ± 7.5) mmol / L and (77.1 ± 9.4) mmol / L, respectively, which were significantly lower than those in control group (424.3 ± 61.2) mmol / L (P <0.01). The level of IL-10 (268.1 ± 24.3) pg / ml and splenic CD4 ~ + CD25 ~ + T lymphocytes (1.25 ± 0.03)% after 1.25 (OH) Compared with the control group [(50.1 ± 3.0) pg / ml and (8.95 ± 2.01)%] (P <0.01). Conclusions 1.25 (OH) _2D_3 can significantly promote the production of IL-10 and the proliferation of CD4 ~ + CD25 ~ + T cells in vivo, and the combined application of 1,25 (OH) _2D_3 and CsA can significantly improve renal function, Survival time.