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研究了激发子对烟草抗赤星病的诱导作用及诱导烟草几丁质酶、β－１，３－葡聚糖酶的积累情况．结果表明（１）激发子诱导后使烟草抗赤星病的能力提高５５．１３％，比水杨酸诱导效果低２１．８６％；（２）激发子诱导的β－１，３－葡聚糖酶和几丁质酶有明显的体外活性，二者单独使用时，酶活性单位分别为１．１６４和０．３１５ｕ，但二者混合作用时，酶的活性明显下降，活性单位分别为０．１２４和０．０５ｕ；（３）ＰＡＧＥ电泳显示，经激发子诱导后ＰＲ蛋白表达量远远超过对照，但比水杨酸诱导积累弱．可见，ＰＲ蛋白、β－１，３－葡聚糖酶、几丁质酶的积累与抗病诱导效果呈一致趋势，表明激发子诱导的烟草抗赤星病的作用可能与ＰＲ蛋白积累及这两种酶的诱导表达有关．
The induction of elicitor on tobacco against brown spot disease and the accumulation of tobacco chitinase and β-1, 3-glucanase were studied. The results showed that: (1) the ability of tobacco to induce brown spot resistance was increased by 55.13% after induced by elicitor, which was 21.86% lower than that induced by salicylic acid; (2) induced by β-1,3-glucan Enzymes and chitinase have obvious in vitro activity. When used alone, the enzyme activity units are 1.164 and 0.315u, respectively. However, when the two are mixed, the activity of enzyme decreases obviously, and the activity unit is 0 respectively. 124 and 0.05u; (3) PAGE electrophoresis showed that the expression of PR protein induced by the elicitor far exceeded that of the control, but the accumulation was weaker than that induced by salicylic acid. It can be seen that the accumulation of PR protein, β-1,3-glucanase and chitinase shows a consistent trend with the disease-resistant inducing effect, indicating that the effect of the elicitor-induced tobacco on the resistance to brown spot disease may be related to the PR protein accumulation and both The induction of the expression of the enzyme.