论文部分内容阅读
本文报道用尼龙网作载体,用抗乙型肝炎表面抗原作模型抗体,采用三种不同方法制作抗体膜,并在理论上进行了讨论.结果证明用硫酸二甲酯,1,6—己二胺和戊二醛制作的抗体膜最适于组装酶免疫传感器.因为它具有机械强度高、抗体结合量大,空间位阻小和气液通透性好等特点.酶免疫传感器的响应时间和电极上没有抗体膜时对酶反应产物的响应时间一样.
This article reports the use of nylon mesh as a carrier, with anti-hepatitis B surface antigen as a model antibody, the use of three different methods for the production of antibody membrane, and in theory were discussed. The results show that with dimethyl sulfate, 1,6- Amine and glutaraldehyde antibody film is most suitable for the assembly of enzyme immunoassay sensor because of its high mechanical strength, antibody binding capacity, steric hindrance and gas-liquid permeability and good characteristics of the enzyme immunosensor response time and electrode There is no antibody membrane on the reaction time of the enzyme reaction product.