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在加拿大,加籍华人何克明技师,采用剪雄杂交水培法,进行大麦杂交育种,取得了很好的效果。具体做法是:从田间亲本圃里,把开花前一天的亲本(父本和母本)植株从地平面剪下来,插在准备好的液体培养基中,带回实验室对母本植株进行“剪颖去雄”(将穗倒立,剪掉颖片和雄蕊),从顶部往下留三片叶子,然后把母本穗和父本穗用透明塑料袋套在一起,插入准备好的B_5培养基中,放进温室里。第二天或第三天雄蕊吐丝后,用手轻拍穗子,使其传粉,一个月就能收到种子。在这期间每星期要更换1—2次培养基。
In Canada, Chinese-Canadian He Keming technician, using the method of male crossbreed hydroponics, for barley cross breeding, and achieved good results. The specific practice is: from the field of parental nursery, the day before flowering of the parent (male and female) plants cut from the ground plane, inserted in the prepared liquid medium, back to the laboratory maternal plant “ Cut the Ying to the male ”(the spike inverted, cut the glumes and stamens), leaving three leaves from the top down, and then the mother of the ear and the parent panicle with a transparent plastic bag together, into the prepared B_5 culture Base, into the greenhouse. The next day or the third day after the stamens silt, tap the ears by hand to pollinate and receive the seeds in a month. During this period to be replaced 1-2 times a week medium.