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用同步荧光光谱法研究细胞色素Ｃ在水溶液中的存在形态，发现细胞色素Ｃ中色氨酸残基的同步荧光光谱随浓度改变而发生变化，在较稀的溶液中，只观察到色氨酸残基的一个荧光峰（３４０．０ｎｍ）；在较浓的溶液中也观察到一个荧光峰（３０４．０ｎｍ）；而在这两个浓度之间时，这两个峰共存，可能反映了细胞色素Ｃ在不同浓度溶液中聚集状态的不同．当向存在两个荧光峰的溶液中加入不同浓度的脲，在一定脲浓度范围内，细胞色素Ｃ不发生变性．脲的作用使细胞色素Ｃ的单体浓度增加，聚集体浓度降低．
Simultaneous fluorescence spectroscopy was used to study the existence of cytochrome C in aqueous solution. It was found that the synchronous fluorescence spectra of tryptophan residues in cytochrome C changed with the change of concentration. Only tryptophan was observed in the more dilute solution (340.0 nm) of the residue; a fluorescence peak (304.0 nm) was also observed in the more concentrated solution; and at these two concentrations, the two peaks coexisted, possibly reflecting the cell Pigment C concentration in different concentrations of different aggregation state. When different concentrations of urea were added to the solution with two fluorescence peaks, cytochrome C did not degenerate within a certain urea concentration range. The action of urea increases the monomer concentration of cytochrome C and decreases the concentration of aggregates.