论文部分内容阅读
目的:研究转化生长因子(TGF-β1)-509(C/T)基因多态性与子宫内膜异位症易感性的关系。方法:采用PCR-RFLP法和PCR产物直接测序法,检测80例子宫内膜异位症和80例正常对照组TGF-β1-509(C/T)基因型及等位基因分布。结果:子宫内膜异位症组TGF-β1-509CC、CT、TT基因型频率分别为20%、35%、45%,C和T等位基因频率分别为37.5%和62.5%;对照组中TGF-β1-509CC、CT、TT基因型频率分别为30%、32.5%、37.5%,C和T等位基因频率分别为46.25%和53.75%。基因型频率和等位基因频率在两组之间无显著性差异(P>0.05、P>0.05)。结论:TGF-β1-509(C/T)基因多态性与子宫内膜异位症无关联。
Objective: To investigate the relationship between polymorphism of transforming growth factor-β1 (TGF-β1) -509 (C / T) gene and susceptibility to endometriosis. Methods: The genotype and allele distribution of TGF-β1-509 (C / T) in 80 cases of endometriosis and 80 cases of normal controls were detected by PCR-RFLP and PCR products. Results: The frequencies of TGF-β1-509CC, CT and TT genotypes in endometriosis group were 20%, 35% and 45%, respectively. The frequencies of C and T alleles were 37.5% and 62.5% respectively. In the control group The frequencies of TGF-β1-509CC, CT and TT genotypes were 30%, 32.5% and 37.5%, respectively. The frequencies of C and T alleles were 46.25% and 53.75%, respectively. There was no significant difference in genotype frequency and allele frequency between the two groups (P> 0.05, P> 0.05). Conclusion: There is no correlation between TGF-β1-509 (C / T) gene polymorphism and endometriosis.