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来源于Cohn’s组分Ⅱ的人标准免疫球蛋白(SIG)用于静脉注射可能会引起严重的不良反应,包括血压下降甚至休克。以往将这类反应归咎于分离过程中形成的IgG聚合物,近来又提出其他原因,如产品中前激肽释放酶原激活剂(PKA)的存在等。作者提出戊巴比妥麻醉鼠模型可为静脉注射用Ig制品的血管活性试验提供一种敏感的可重复的检测系统。方法1.动物模型:重200—250g的雌性Wistar鼠按50mg/kg腹腔注射戊巴比妥麻醉,
Human standard immunoglobulin (SIG), which is derived from Cohn’s Component II, for intravenous injection may cause serious adverse reactions, including decreased blood pressure and even shock. In the past, this type of reaction was attributed to the formation of IgG polymer during the separation. Other reasons have been proposed recently, such as the presence of prekallikrein (PKA) in the product. The authors propose that a pentobarbital anesthetized rat model may provide a sensitive, repeatable detection system for vasoactive assays of intravenous Ig products. Methods 1. Animal models: Female Wistar rats weighing 200-250 g were anesthetized with 50 mg / kg intraperitoneal injection of pentobarbital,