目的观察重组日本血吸虫中国大陆株脂肪酸结合蛋白（ｒＳｊ１４ＦＡＢＰ）免疫动物所诱导的免疫保护效果。方法用ｒＳｊ１４ＦＡＢＰ分别对昆明系小鼠、Ｗｉｓｔａｒ大鼠及绵羊进行３次免疫，于第３次免疫后２周，分别用４０、６００、及３００条尾蚴攻击，于攻击后６～８周剖杀小鼠和大鼠，１１周剖杀绵羊，观察各实验组的减虫率及绵羊免疫组的组织、粪便减卵率。结果在小鼠试验中，以ＢＣＧ为佐剂进行皮内免疫获得３３． ７％的减虫率（Ｐ＜０．０５），明显高于以 ＦＣＡ／ＦＩＡ为佐剂进行肌肉免疫所获得的减虫率４．７％（Ｐ＞０．０５）。用ｒＳｊ１４ＦＡＢＰ皮内免疫大鼠，获得的减虫率为３１．９％，但和对照组相比无显著性差异。用ｒＳｊ１４ＦＡＢＰ皮内免疫绵羊，减虫率为５９．２％（Ｐ＜０．０１），同时，自攻击后第６周至１１周，绵羊免疫组粪便中平均每克虫卵数（ＥＰＧ）分别比对照组减少２３．６％～６３．３％，第１１周解剖时，肝组织ＥＰＧ减少４４．９％，小肠ＥＰＧ减少６９．６％，大肠ＥＰＧ未见减少。结论ｒＳｊ１４ＦＡＢＰ能诱导抗日本血吸虫攻击感染的部分保护作用，值得作为抗日本血吸虫候选疫苗抗原进一步探讨。 Objective To observe the immunoprotective effect induced by rSj14FABP immunized animal of recombinant Schistosoma japonicum in Chinese mainland. Methods Kunming mice, Wistar rats and sheep were immunized three times with rSj14FABP. After 40 days, 40 and 600 and 300 cycles of cercariae respectively after the third immunization, the mice were killed at 6-8 weeks after challenge Mice and rats were killed on the 11th week. The worm reduction rates of the experimental groups and the tissue and feces reduction rates of the immunized groups were observed. Results In a mouse trial, intradermal immunization with BCG was obtained 33. 7% (P <0.05), which was significantly higher than that of mice immunized with FCA / FIA as adjuvant (P> 0.05). Rats immunized intradermally with rSj14FABP achieved an MDR of 31.9%, but no significant difference from the control group. The intradermal immunization of sheep with rSj14FABP reduced the worm reduction rate to 59.2% (P <0.01). At the same time, the average number of eggs per gram of eggs (EPG) in the sheep immunized group was respectively The control group decreased by 23.6% -63.3%. In the 11th week, the liver tissue EPG decreased by 44.9%, the small intestine EPG decreased by 69.6%, and the large intestine EPG was not reduced. Conclusion rSj14FABP can induce part of the protective effect against Schistosoma japonicum infection and is worth further investigation as a vaccine antigen against Schistosoma japonicum.