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小卫星DNA探针MZ1.3是从人类基因库中通过筛选随机选择克隆中的超可变重复序列而分离的。详细制备方法及该选择性DNA克隆序列的细节将另行发表。自外周血细胞及尸解材料组织标本抽提的人DNA用限制性内切酶HinfI酶解,再作Southern印迹法分析。在与经~(32)P-dCTP缺口翻译的探针MZ1.3杂交后,经放射性自显影,每一个体DNA样本平
The small satellite DNA probe MZ1.3 is isolated from the human gene pool by screening for the hypervariable repeat sequences in the randomly selected clones. Details of the method of preparation and details of the selective DNA cloning sequence will be published separately. Human DNA extracted from peripheral blood and autopsy tissue samples was digested with restriction endonuclease HinfI and analyzed by Southern blotting. After hybridization with probe MZ1.3, which has been nicked by ~ (32) P-dCTP, autoradiography was performed, and each individual DNA sample was flat