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目的:探讨再矿化介质聚乙烯磷酸和聚丙烯酸对3种粘结剂(Fluoro Bond II、Xeno-III和i Bond)和牙本质所形成的树脂-牙本质界面再矿化程度的影响。创新点:(1)多数仿生再矿化研究集中于通过透射电子显微镜观察牙本质粘结剂界面的再矿化情况,鲜有采用激光共聚焦显微镜(CLSM)作为观察手段的。虽有研究应用CLSM,但也仅是从定性的角度比较再矿化效应,没有定量分析比较,难以令人信服。而本项研究从定性和定量两方面分析对比。(2)本研究从当前牙本质粘结剂系统(共七代)常用的后四代中选择典型代表产品为研究对象,便于横向比较各类粘结剂。(3)本研究的定量结果除了比较同一时间实验组和对照组的矿化情况,还增加了纵向分析思路,进一步比较矿化程度随时间的变化以及不同牙本质粘结剂粘结界面的矿化情况。方法:将96颗健康前磨牙按照Fluoro Bond II、Xeno-III和i Bond粘结剂随机分为3组,每颗牙均暴露表层牙本质。3种粘结剂分别严格按照各自产品说明书处理牙本质表面,牙合面堆制5 mm厚的树脂。每颗牙沿牙合-龈向切成0.9 mm厚的薄片,获取中间两片样本用于矿化组和模拟组。矿化组采用含聚乙烯磷酸和聚丙烯酸的再矿化液浸泡;模拟组采用不含聚乙烯磷酸和聚丙烯酸的模拟体液浸泡。各组标本在储存1、2、3和4个月后,各取出8片,经苏丹明B荧光染料染色24 h,冲洗,吹干,置CLSM下观察渗入混合层及粘结层的荧光情况,测量荧光面积、平均荧光量及总荧光量。所有数据统计方法采用方差分析(ANOVA)和Tukey’s HSD检验分析。结论:本研究中聚丙烯酸和聚乙烯磷酸双仿生类似物分子对Fluoro Bond II、Xeno-III及i Bond粘结剂均显示不同程度的再矿化效应,其中对i Bond粘结剂再矿化效应最明显,Fluoro Bond II粘结剂次之,Xeno-III粘结剂再矿化效应较差,但能够起到抵制基底矿物继续丢失或阻止胶原降解的作用。CLSM结合应用苏丹明B是量化混合层再矿化的一项有效手段。因此,上述双仿生类似物分子应用于口腔粘结剂修复材料促使混合层再矿化,具有良好的应用前景,值得进一步深入研究。
OBJECTIVE: To investigate the effect of remineralization medium of polyethylene phosphate and polyacrylic acid on the remineralization of three dentin interface formed by Fluoro Bond II (Xeno-III and i Bond) and dentin. Innovations: (1) Most bionic and remineralization studies focus on the remineralization of the dentin binder interface by transmission electron microscopy, and few use of confocal laser scanning microscopy (CLSM) as a means of observation. Although CLSM has been studied, the remineralization effect is only compared qualitatively. It is hard to be convincing without quantitative analysis and comparison. However, this study analyzes and contrasts qualitatively and quantitatively. (2) In the present study, typical representative products were selected from the last four generations of the current dentin binder system (seven generations in total) as the research object to facilitate the horizontal comparison of various binders. (3) In addition to comparing the mineralization of the experimental group and the control group at the same time, the quantitative results of this study also increased the longitudinal analysis train of thought, and further compared the mineralization degree with time and the different dentin binder bonding interface The situation. Methods: Ninety-six healthy premolar teeth were randomly divided into three groups according to Fluoro Bond II, Xeno-III and i Bond adhesive. Each tooth exposed the top dentin. Three kinds of binders were used to treat the dentin surfaces in strict accordance with the respective product specifications. The occlusal surfaces were made of 5 mm thick resin. Each tooth was cut into a 0.9 mm thick piece along the occlusal-gingivae and two middle pieces were taken for mineralization and simulation. The mineralization group was immersed in remineralized solution containing poly (ethylene-co-phosphoric acid) and poly (acrylic acid). The simulated group was immersed in simulated body fluid without poly (ethylene phosphate) and poly (acrylic acid). Eight samples were taken from each group after storage for 1, 2, 3 and 4 months, stained with Sudan B fluorescent dye for 24 hours, rinsed, dried and clouded to observe the fluorescence of the mixed layer and the adhesive layer by CLSM , Measuring the fluorescence area, the average fluorescence amount and the total fluorescence amount. All statistical methods were analyzed by analysis of variance (ANOVA) and Tukey’s HSD test. CONCLUSION: Biomimetic analogue molecules of polyacrylic acid and polyvinylacetic acid show a different degree of remineralisation on Fluoro Bond II, Xeno-III and i Bond adhesives in this study, with i Bond binder remineralization The effect is most pronounced, followed by Fluoro Bond II binder, and Xeno-III binder has less remineralization but can resist the loss of base minerals or prevent collagen degradation. CLSM in combination with Sudan B is an effective method to quantify remineralization of mixed layers. Therefore, the application of the above biomimetic analogue molecule to the oral adhesive repair material to promote remineralization of the mixed layer has a good application prospect and deserves further study.