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免疫层析技术是一种建立在层析技术和抗原抗体特异性免疫反应基础上的免疫检测技术。近年来国内外食源性疾病频繁爆发,越来越引起社会的关注。因此建立一种快速、简便且适用于食源性病原菌的检测方法在预防和诊断食源性疾病中具有重大的社会意义。免疫层析技术具有灵敏度高、特异性强、操作简便及检测速度快等特点,已经被广泛应用于食品领域的检测中。本文综述了6种免疫标记材料在检测不同种类病原菌方面的应用,并对其检测特点进行了介绍。
Immunochromatography is an immunoassay based on chromatographic techniques and antigen-antibody-specific immunoreactions. In recent years, frequent outbreaks of food-borne diseases at home and abroad have drawn more and more attention from the community. Therefore, it is of great social significance to establish a rapid, simple and suitable detection method for foodborne pathogens in the prevention and diagnosis of foodborne diseases. Immunochromatography with high sensitivity, specificity, ease of operation and detection speed and other characteristics, has been widely used in the field of food testing. This review summarizes the application of six kinds of immunolabeling materials in the detection of different kinds of pathogens, and introduces the characteristics of their detection.