论文部分内容阅读
在神经解剖HRP法试验过程中,常常需要用微量注射器连通塑料管及玻璃电极进入细胞核团内注射,有的用手慢慢推动微量注射器进行注射,结果注射点过大,HRP扩散,注射时间又难于控制,所以试验易于失败或效果不佳。目前,国内一些院校是利用万用表的测流微安挡,连接电源,一端连接电极夹持针灸针,插入玻璃电极,进行细胞核团内HRP法电泳,一端连在动物的肢体肌肉上(图1),这样,效果较前法为好,但是最大缺
In the process of neuroanatomy HRP test, it is often necessary to use a micro syringe connected to the plastic tube and the glass electrode into the nucleus of the injection, and some slowly push the micro syringe syringe, the injection point is too large, HRP diffusion, injection time Difficult to control, so the test is easy to fail or ineffective. At present, some institutions in our country use the micro-ampere meter of flowmeter to connect the power supply, one end connects the electrode to hold the acupuncture needle, insert the glass electrode, carry out HRP electrophoresis in the cell nucleus, one end is connected with the animal’s limb muscle ), So that the effect is better than the previous method, but the biggest lack