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目的探讨免疫球蛋白重链(IgH)及T细胞受体γ(TCRγ)基因重排在非霍奇金淋巴瘤(nonHodgkin’slymphoma,NHL)临床诊断中的应用。方法用聚合酶链反应(PCR)技术对58例B细胞淋巴瘤(BNHL)、15例T细胞淋巴瘤(TNHL)、不能确诊为NHL的标本5例及10例良性淋巴组织增生标本进行IgH及TCRγ基因重排的检测。结果58例B细胞淋巴瘤标本中有IgH基因重排为47例,有3例TCRγ基因重排。15例T细胞淋巴瘤中TCRγ基因重排为11例,未见有IgH基因重排。5例未确诊的疑难病例均为IgH基因重排。10例良性淋巴组织反应性增生病例中,IgH及TCRγ基因重排均为阴性。IgH及TCRγ基因重排检出率在淋巴瘤组与良性组之间,差异有显著意义(P<0.05)。结论用PCR方法检测IgH及TCRγ基因重排对NHL及其疑难病例有重要的诊断价值。
Objective To investigate the clinical application of immunoglobulin heavy chain (IgH) and T cell receptor γ (TCRγ) gene rearrangements in the diagnosis of non-Hodgkin’s lymphoma (NHL). Methods Fifty-eight patients with B-cell lymphoma (BNHL), 15 T-cell lymphoma (TNHL), 5 with non-diagnosis of NHL and 10 with benign lymphoid hyperplasia were detected by polymerase chain reaction (PCR) Detection of TCRy gene rearrangements. Results There were 47 cases of IgH gene rearrangement in 58 cases of B cell lymphoma and 3 cases of TCRγ gene rearrangement. TCRγ gene rearrangements in 15 cases of T-cell lymphoma were found in 11 cases, and no IgH gene rearrangement was found. Five cases of undiagnosed difficult cases are IgH gene rearrangement. In 10 cases of benign lymphoid reactive hyperplasia, IgH and TCRγ gene rearrangements were negative. The detection rate of IgH and TCRγ gene rearrangements was significantly different between lymphoma group and benign group (P <0.05). Conclusions The detection of IgH and TCRγ gene rearrangements by PCR has important diagnostic value for NHL and its difficult cases.