【摘 要】
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OBJECTIVE:To evaluate the protective efficacy of Sanqi(Radix Notoginseng)on cerebral hemorrhage in a rat model of traumatic brain injury(TBI)by in-vestigating p
【机 构】
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Department of Neurosurgery of the First Affiliat-ed Hospital of Zhejiang Chinese Medical University,
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OBJECTIVE:To evaluate the protective efficacy of Sanqi(Radix Notoginseng)on cerebral hemorrhage in a rat model of traumatic brain injury(TBI)by in-vestigating plasminogen activator inhibitor-1(PAI-1),tissue-type plasminogen activator(t-PA),nuclear factor-KB(NF-κB,p-p65),nitric oxide(NO),endothelin(ET),cluster differentiation(CD61CD62),and coagulation.METHODS:The free-fall method was used to cre-ate a rat model of TBI.Forty-eight rats were ran-domly divided into six groups:the blank group,sham group,model group,low-dose Sanqi(Radix Notoginseng)group,middle-dose Sanqi(Radix No-toginseng)group,and high-dose Sanqi(Radix No-toginseng)group.At 24 h after the model was creat-ed,we investigated brain MRI,brain tissue morphol-ogy using HE staining,flow cytometry,and immu-nohistochemical changes.RESULTS:Cerebral hemorrhage was aggravated in TBI rats(observed in brain specimens,brain MRI,and brain tissue HE).Cerebral immunohistochemis-try results demonstrated that the expression of t-PA,PAI-1 and p-p65 increased significantly in TBI rats,while t-PA/PAI-1 had a significant decrease.In addition,CD61CD62,D2D,and ET were significant-ly increased in TBI rats,and PT and APTT were sig-nificantly prolonged;in contrast,NO was signifi-cantly decreased.Sanqi(Radix Notoginseng)de-creased cerebral hemorrhage in TBI rats(observed in brain MRI and brain tissue HE),and increased t-PA/PAI-1,CD61CD62 significantly.It also signifi-cantly decreased the expression of t-PA,PAI-1,and p-p65 in brain immunohistochemistry and signifi-cantly decreased PT,APTT,D2D,and ET.However,there were no differences in NO between the mod-el group and the Sanqi(Radix Notoginseng)group.CONCLUSION:Sanqi(Radix Notoginseng)can de-crease the expression of p-p65,increase t-PA/PAI-1,and stem traumatic intracranial hemorrhage in a TBI rat model.
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