最近,新西兰试用组织培养法繁殖黑杨派和青杨派杨树获得成功。具体作法是: 从钻天杨、美洲黑杨与欧洲黑杨杂交种“弗累瓦杨”(P.Flevo)和滇杨的发叶枝或休眠枝上切取腋芽,浸泡于乙醇溶液中进行表面消毒,用火烤一下,再浸于0.2%次氯盐酸溶液中,然后用无菌水冲洗数次,剥掉外包皮后置于培养基(0.2%苯甲萘)中,温度保持25℃,光照16小时,总辐射通量密度20瓦/米~2。腋芽在2～3周内开 Recently, the New Zealand Trial Tissue Culture Popularized Black Poplars and Poplar Poplars for success. The specific practice is: cut axillary buds from the leaves or dormant branches of P. aeruginosa Populus and Populus hybrids “P.Flevo” and Populus euphratica, soaked in ethanol solution for surface disinfection with Roasted, and then immersed in 0.2% hydrochloric acid solution, and then rinsed several times with sterile water, peeled off the outer layer of the culture medium (0.2% benzil), the temperature was maintained at 25 ℃, light 16 hours , The total radiation flux density of 20 watts / m ~ 2. Axillary buds open in 2 to 3 weeks