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采用 ＰＣＲ－ＳＳＣＰ－ＥＢ染色法及免疫组化技术检测 １４例食管癌手术切除组织中Ｐ５３基因 ５－８外显子基因突变及Ｐ５３蛋白的表达。结果：其中 ７例免疫组化结果阳性， ６例检出Ｐ５３基因突变。基因及Ｐ５３突变。Ｐ５３的基因突变和过度表达在食管癌的发生发展中发挥重要作用，Ｐ５３基因突变／过度表达可以作为判断食管癌发生及预后的一个指标。
The mutations of P53 gene 5-8 exon and the expression of P53 protein in 14 cases of esophageal carcinoma were detected by PCR-SSCP-EB and immunohistochemistry. Results: Immunohistochemical results were positive in 7 of them and mutation of P53 was detected in 6 of them. Gene and P53 mutations. P53 gene mutation and overexpression plays an important role in the occurrence and development of esophageal cancer. The mutation / overexpression of P53 gene can be used as an index to judge the occurrence and prognosis of esophageal cancer.