,PUB7, a pollen expression gene induced by self-pollination, negatively regulates pollen germination

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Self-incompatibility (SI) is a widespread reproductive mechanism that prevents inbreeding by rejecting self-pollination in plants.In Brassica,the recognition between pollen and stigma is highly specific,and requires species-specific protein interactions [1].Selfincompatibility is regulated by S-locus cysteine-rich protein (SCR),a stamen pollen response factor,which interacts with S-locus receptor kinase (SRK) and results in pollen rejection [2,3].Armadillo repeat containing 1 protein (ARC1) is a downstream component of SRK,which is located in the cytoplasm,and is phosphorylated by SRK.In the downstream signaling of self-pollination,ARC1 combines with Exo70A1 and forms a protein complex composed of ubiquitinated Exo70A1 complexed with ubiquitin-activating (E1) and ubiquitinconjugating (E2) enzymes.Subsequently,a 26 S proteasome/COP9 signalosome triggers Exo70A1 degradation,leading to the inhibition of pollen germination in the stigma [4].
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