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采用典型的籼粳交、ZYQ8/JX1 7的DH群体为材料 ,在小型人工气候箱中对水稻苗期的耐冷性进行了研究 .发现该DH群体的籼、粳双亲苗期耐冷性存在明显差异 ;整个群体苗期耐冷性动态分析表明 :冷处理的第 6 ,7d ,受冷害伤害致死的株系迅速上升 ,而此前的低温处理和此后的常温恢复的株系死苗数表现相对较少 .用该群体构建的分子连锁图谱进行了苗期耐冷性逐日数量性状座位 (QTLs)的分析 ,在第 1 ,2 ,3,4染色体上分别检测到与苗期耐冷性有关的 4个QTLs ,第 1 ,2 ,3染色体qSCT 1 ,qSCT 2 ,qSCT 3的加性效应均为正 ,即来自JX1 7的QTLs对耐冷性表现影响大 ,位于第 4染色体qSCT 4的加性效应为负 ,即来自ZYQ8的QTLs对耐冷性表现影响大 ,4个QTLs表现时间不同 ,显示了耐冷性遗传机制的复杂 .进一步对DH群体中几个超亲分离的株系耐冷性QTLs基因型进行分析 ,发现超耐冷的带有 4个或 3个QTLs ,这种耐冷性QTLs聚合、重组可为育种提供选择依据 .
Cold tolerance of rice at seedling stage in a small artificial climatic chamber was studied using a typical DH population from indica and japonica hybrids ZYQ8 / JX1 7. The results showed that there was a significant difference in cold tolerance between indica and japonica parents at seedling stage The dynamic analysis of seedling chilling tolerance in the whole population showed that the number of cold-injured plants on the 6th and 7th day increased rapidly after chilling injury, while the number of dead seedlings in the previous low temperature treatment and subsequent normal temperature recovery was relatively low. The QTLs for cold tolerance at seedling stage were analyzed by the molecular linkage map constructed in this population. Four QTLs related to cold tolerance at seedling stage were detected on chromosomes 1, 2, 3 and 4 respectively. The first The additive effects of qSCT 1, qSCT 2 and qSCT 3 on chromosome 2 and 3 were all positive, that is, the QTLs derived from JX1 7 had a significant effect on cold tolerance and the additive effect on qSCT 4 on chromosome 4 was negative, namely, from ZYQ8 QTLs had a great effect on cold tolerance and four QTLs showed different time, which indicated the complexity of the genetic mechanism of cold tolerance.Furthermore, we analyzed the QTLs of several cold tolerance genes in DH population, With 4 or 3 QTLs, This cold tolerance QTLs aggregation, recombination can provide a basis for breeding selection.