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目的通过对广西市售速冻米面制品中检出的86株金黄色葡萄球菌进行肠毒素分型和耐药性检测,了解其产肠毒素特性和耐药性。方法依据GB4789.10-2010采用ELISA方法测定金黄色葡萄球菌肠毒素SEA-SEE,利用微量肉汤法对菌株进行药敏试验。结果 86株金黄色葡萄球菌中有47株肠毒素阳性,产肠毒素阳性率为54.65%,产2种以上肠毒素的有26株,在产毒株中占55.32%,β-内酰胺酶阳性金黄色葡萄球菌对青霉素(95.92%)四环素(85.71%)、氯霉素(81.63%)、红霉素(77.55%)耐药较为严重,β-内酰胺酶阳性金黄色葡萄球菌整体耐药性显著高于β-内酰胺酶阴性株。结论食源性金黄色葡萄球菌产毒素能力较强,对于金黄色葡萄球菌的感染治疗应根据药敏结果选择合理的抗生素。
Objective To detect the staphylococcal enterotoxigenicity and drug resistance of 86 Staphylococcus aureus isolates detected from the commercially available frozen rice noodles in Guangxi Province. Methods Staphylococcus aureus enterotoxin SEA-SEE was determined by ELISA according to GB4789.10-2010, and antibiotic susceptibility test was carried out on the strain by using micro broth method. Results Among the 86 Staphylococcus aureus isolates, 47 were positive for enterotoxigenin, 54.65% for enterotoxigenic, 26 were for 2 or more enterotoxins, 55.32% were for toxigenic strains and were positive for β-lactamase Staphylococcus aureus was more resistant to penicillin (95.72%), tetracycline (85.71%), chloramphenicol (81.63%) and erythromycin (77.55%), and the overall resistance to β-lactamase-positive Staphylococcus aureus Significantly higher than the β-lactamase-negative strain. Conclusion Food-borne Staphylococcus aureus toxin production capacity of strong, for the treatment of Staphylococcus aureus infection should be based on susceptibility results to choose a reasonable antibiotics.