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选用白细胞粘附抑制试验来鉴定乙肝特异性转移因子(HBV-TF)免疫活性。实验结果表明:经HBV-TF致敏的淋巴细胞与乙肝抗原(HBV-Ag)孵育后,显示出明显的粘附抑制活性,而非特异性转移因子(TFn)致敏淋巴细胞与乙肝抗原孵育、HBV-TF致敏淋巴细胞与甲肝抗原(HAV-Ag)孵育或HBV-TF致敏淋巴细胞与1640液(代替抗原液)孵育时均不能表现出这种抑制作用,说明HBV-TF具有抗原特异性免疫活性。实验证明,本法是体外鉴定HBV-TF免疫活性简便易行的方法。
The leukocyte adhesion inhibition assay was used to identify the hepatitis B-specific transfer factor (HBV-TF) immunocompetence. The experimental results showed that the lymphocytes sensitized by HBV-TF showed obvious adhesion inhibition activity after incubation with hepatitis B antigen (HBV-Ag), whereas the TFn-sensitized lymphocytes incubated with hepatitis B antigen, HBV-TF sensitized lymphocytes incubated with hepatitis A antigen (HAV-Ag) or HBV-TF-sensitized lymphocytes incubated with 1640 solution (instead of antigen solution) can not show this inhibitory effect, indicating that HBV-TF has antigen specificity Immune activity. Experiments show that this method is in vitro identification of HBV-TF immune activity simple and easy method.