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目的：观察蒺藜总皂甙（ＧＳＴＴ）对牛脑微血管内皮细胞（ＢＣＭＥＣ）表达Ｅ选择素的影响。方法：流式细胞仪测定白细胞介素１β（ＩＬ－１β）诱导ＢＣＭＥＣ粘附大鼠血单核细胞（ＭＮＣ）和中性粒细胞（ＮＴＰ）的数目；应用ＥＬＩＳＡ法检测ＢＣＭＥＣ表达Ｅ选择素的量。结果：ＢＣＭＥＣ经ＩＬ－１β诱导后，明显增加细胞表面Ｅ选择素的表达；ＢＣＭＥＣ与白细胞的粘附率亦明显增加，对ＭＮＣ的粘附率从（１２．９±０．４）％增至（４５．７±０．６）％，对ＮＴＰ的粘附率从（１４．７±０．３）％增至（４４．９±０．６）％。抗Ｅ选择素单克隆抗体（ＡＥｍＡｂ）能显著降低ＩＬ－１β的诱导作用。在ＩＬ－１β处理前，用ＧＳＴＴ（０．０１～１．００ｇ／Ｌ）与ＢＣＭＥＣ共孵育，则ＧＳＴＴ可浓度依赖性地抑制ＩＬ－１β的诱导作用，降低Ｅ选择素的表达量。结论：ＧＳＴＴ能抑制ＢＣＭＥＣ表达Ｅ选择素
Objective: To observe the effect of gross saponin tribulus terrestris (GSTT) on the expression of E-selectin in bovine microvascular endothelial cells (BCMEC). Methods: The numbers of monocyte (MNC) and neutrophil (NTP) in the blood of BCMEC-adherent rats induced by interleukin-1β were detected by flow cytometry. The expression of E-selectin the amount. Results: After induced by IL-1β, BCMEC significantly increased the expression of E-selectin on the cell surface. The adhesion rate of BCMEC to leukocytes was also significantly increased, and the adhesion rate to MNC increased from (12.9 ± 0.4)% to (45.7 ± 0.6)%, and the adhesion rate to NTP increased from (14.7 ± 0.3)% to (44.9 ± 0.6)%. Anti-E-selectin monoclonal antibody (AEmAb) significantly reduced the induction of IL-1β. GSTT co-incubated with BCMEC with GSTT (0.01 ~ 1.00g / L) before IL-1β treatment inhibited the induction of IL-1β in a concentration-dependent manner and decreased the expression of E-selectin. Conclusion: GSTT can inhibit the expression of E-selectin in BCMEC