为配合临床寻找口服棉酚最小有效剂量,探索棉酚对人体抑精的作用部位,在常规精液检查的同时,对服药前、后的精子作细胞学,细胞化学,及电镜的综合观察,为棉酚的临床应用提供依据。实验分城市及农村两个临床服药组,在服药前,服药后2、4、6、8周(城市)及3、6周(农村)分别收集精液,于2小时内作细胞学检查用甲醇固定,H.E 染色细胞化学;琥珀酸脱氢酶(SDH)及溶旦白酶(PLE)。供电镜观察材料包埋于618树 In line with the clinical search for the minimum effective dose of oral gossypol, gossypol on human body to explore the role of inhibition of the site, in the conventional semen examination at the same time, before and after medication for cytology, cytochemistry, and electron microscopy of the comprehensive observation of Gossypol clinical basis to provide. The experiment was divided into two clinical medication groups in urban and rural areas: semen was collected before taking medicine, at 2, 4, 6 and 8 weeks (city) and 3 and 6 weeks (rural area) after taking medicine, and cytology was performed within 2 hours with methanol Fixed, HE-stained cytochemistry; succinate dehydrogenase (SDH) and lysozyme (PLE). Power microscope observation material embedded in the 618 tree