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应用6-羟多巴胺(6-OHDA)损毁 SD 大鼠单侧黑质制备偏侧帕金森病(PD)鼠模型。3周后根据药物诱发试验,酪氨酸羟化酶(TH)免疫组化证实模型制作成功。并用脑微透析技术结合 HPLC-ECD 在体检测 PD 鼠纹状体多巴胺及代谢产物含量。结果:82只大鼠中有36只阿朴吗啡诱发的旋转次数>7r/min。6-OHDA 注射侧黑质 DA 神经末稍已绝大多数被损毁。6-OHDA 损毁侧纹状体多巴胺及代谢产物明显低于健侧(P<0 05,P<0.01)。结论认为,应用6-OHDA 制备的偏侧 PD 鼠模型是 PD 研究的理想模型之一。
A rat model of unilateral Parkinson’s disease (PD) was established by 6-hydroxydopamine (6-OHDA) destroying unilateral substantia nigra of SD rats. Three weeks later, according to the drug-induced test, tyrosine hydroxylase (TH) immunohistochemistry confirmed that the model was successfully made. The contents of dopamine and its metabolites in striatum of the striatum of rats were measured by brain microdialysis combined with HPLC-ECD. RESULTS: Thirty-six apomorphine-induced rotations were> 7 r / min in 82 rats. 6-OHDA injection side of the substantia nigra DA nerve endings have been the vast majority of damage. 6-OHDA lesioned striatum dopamine and metabolites were significantly lower than the contralateral side (P <0.05, P <0.01). It is concluded that the hemiparkinsonian PD model prepared by 6-OHDA is one of the ideal models for PD study.