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目的观察大鼠脑缺血后少突胶质前体细胞(oligodendrocyte progenitor cells,OPC)的表达,并初步探讨血管内皮生长因子(vascular epithelial growth factor,VEGF)在其中的作用。方法实验动物72只,按随机数字表法分为假手术对照组、缺血组和缺血+贝伐单抗组共3组,每组设6、12、24、72 h 4个时相点,每个时相点6只大鼠,采用激光共聚焦观察缺血后不同时相点NG2和VEGF免疫荧光标记的细胞增殖状况。结果在大脑皮层区,NG2与VEGF标记细胞基本完全重叠。在缺血后72 h内,阳性标记细胞随时间延长,进行性增多,其中贝伐单抗组在各时相点的双阳性细胞数均明显少于缺血组(P<0.05)。结论脑缺血损伤后脑皮层区存在OPC的活化现象。VEGF可能对损伤后NG2的增殖有促进作用。
Objective To observe the expression of oligodendrocyte progenitor cells (OPC) after focal cerebral ischemia in rats and to explore the role of vascular endothelial growth factor (VEGF) in it. Methods Totally 72 experimental animals were divided into sham operation control group, ischemic group and ischemia + bevacizumab group according to random number table method. There were 4 time points of 6, 12, 24 and 72 h in each group , Each time point of 6 rats, using laser confocal laser scanning microscope at different time points after NG2 and VEGF immunofluorescence labeled cell proliferation. Results In the cerebral cortex, NG2 and VEGF labeled cells almost completely overlap. Within 72 hours after ischemia, the number of positive labeled cells increased with time, and the number of double positive cells in the bevacizumab group at each time point was significantly less than that in the ischemic group (P <0.05). Conclusion There is an activation of OPC in cerebral cortex after cerebral ischemic injury. VEGF may promote the proliferation of NG2 after injury.