论文部分内容阅读
目的 :研究肿瘤坏死因子α(ＴＮＦ)引起微血管内皮细胞凋亡的特点及ＦＡＳ ,ＮＦ -κＢ和Ｃａｓｐａｓｅｓ在此种凋亡中的作用。方法 :用ＴＵＮＥＬ ,流式细胞仪等方法测定ＴＮＦ引起体外培养的大鼠肺微血管内皮细胞(内皮细胞 )凋亡。用免疫组化方法测定ＴＮＦ引起内皮细胞ＮＦ
Objective: To investigate the characteristics of tumor necrosis factor-α (TNF) -induced apoptosis of microvascular endothelial cells and the role of FAS, NF-κB and Caspases in this apoptosis. Methods: TUNEL and flow cytometry were used to determine the apoptosis of rat pulmonary microvascular endothelial cells (endothelial cells) in vitro induced by TNF. Immunohistochemistry was used to determine the TNF-induced endothelial NF