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目的:探讨三七总皂甙对大鼠脑出血后神经可塑性的影响。方法:制作脑出血模型;将120只大鼠随机分成假手术组、模型组、三七总皂甙治疗组;免疫组化检测Nestin、Shank1表达;电镜观察突触超微结构并定量分析;对大鼠进行神经功能评分。结果:假手术组超微结构正常,模型组神经突触及细胞器溶解破坏,治疗组突触结构趋于正常,与模型组比较突触数量、界面曲率及突触后致密区均增大,突触间隙变窄,差异有统计学意义(P<0.01)。与模型组比较,治疗组Shank1及Nestin蛋白阳性表达升高,差异有统计学意义(P<0.01)。假手术组神经功能基本正常;模型组评分升高;与模型组比较,治疗组评分降低,差异有统计学意义(P<0.01)。结论:三七总皂甙可增强神经可塑性,保护大鼠神经功能。
Objective: To investigate the effect of Panax Notoginseng Saponins on neuroplasticity after intracerebral hemorrhage in rats. Methods: The model of intracerebral hemorrhage was made. 120 rats were randomly divided into sham operation group, model group and Panax notoginseng saponin treatment group. The expression of Nestin and Shank1 were detected by immunohistochemistry. The synaptic ultrastructure was observed by electron microscope and quantified. Rats were scored for neurological function. Results: The ultrastructure of sham-operation group was normal, the synapses of neurons and organelles were dissolved and destroyed in the model group, and the synaptic structure of the treatment group tended to be normal. Compared with the model group, the number of synapses, the interface curvature and the postsynaptic density increased Touch space narrowing, the difference was statistically significant (P <0.01). Compared with the model group, the positive expression of Shank1 and Nestin protein in the treatment group was significantly increased (P <0.01). Compared with model group, the score of treatment group decreased, the difference was statistically significant (P <0.01). Conclusion: Panax notoginseng saponins can enhance neuroplasticity and protect neurological function in rats.