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采用GFP稳定表达的细胞系(293-BAC)接种裸鼠形成移植瘤.取肿瘤组织进行原代培养,通过GFP示踪和细胞形态学变化,观察了肿瘤组织中细胞的生长规律.肿瘤细胞释放并生长在组织块附近,向周围空间延伸.种植时散落的薄层组织细胞则直接贴壁生长.生长的鼠源间质细胞占总细胞量的1%～3%,散在或在组织新生肿瘤细胞外围集中生长.原代培养的细胞在传代5代以后,其中的鼠源细胞消失.经过成瘤和传代过程的肿瘤细胞生长性能稳定、来源纯净,是相关研究的好材料.观察到的细胞生长规律可为移植瘤的相关研究提供参考.
The nude mice were inoculated nude mice with GFP-stable cell line (293-BAC) to form xenografts.The tumor tissues were primary cultured, and the growth of tumor cells was observed by GFP tracing and cell morphological changes. And grow in the vicinity of the tissue block to extend to the surrounding space when planting scattered thin layer of tissue cells directly adherent growth.The growth of murine stromal cells accounted for 1% to 3% of the total cell mass, scattered or in tissue neoplasms Cell periphery concentrated growth.After subculture for 5 generations, the murine cells disappeared.After tumorigenesis and passage, the growth of tumor cells is stable and the source is pure, which is a good material for related research.Observed cells The law of growth can provide reference for the study of xenograft.