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该实验应用L_(929)细胞杀伤法和微量细胞病变抑制法对乙肝病毒标志物阳性肝癌患者血清中肿瘤坏死因子(TNF)和干扰素(IFN)含量进行了检测,并对肝癌患者血清TNF、IFN含量变化与乙肝病毒复制指标HBeAg的关系进行了观察,结果表明肝癌患者血清中TNF活性明显高于对照组(P<0.01),IFN含量明显低于对照组,肝癌患者HBeAg阳性患者TNF活性及IFN含量显著高于HBeAg阴性患者(P<0.01),表明TNF,IFN与HBV复制的活跃程度有关。这与肝癌患者存在免疫监视作用低下,免疫调节功能失调,表现为细胞免疫与体液免疫功能均受抑制的结果是一致的。
In this experiment, L_(929) cell killing method and micro-cytopathic inhibition method were used to detect the serum levels of tumor necrosis factor (TNF) and interferon (IFN) in patients with hepatitis B virus positive liver cancer. The relationship between IFN content and HBeAg replication index was observed. The results showed that the serum TNF activity in liver cancer patients was significantly higher than the control group (P <0.01), IFN content was significantly lower than the control group, HBeAg positive patients with liver cancer TNF activity and The IFN content was significantly higher than that of HBeAg negative patients (P<0.01), indicating that TNF and IFN are related to the degree of HBV replication activity. This is consistent with the fact that liver cancer patients have low immunosurveillance and dysregulation of immune regulation, which is consistent with the result that both cellular immunity and humoral immunity are inhibited.