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目的 探讨Bcl 2反义基因在单核白血病细胞系U937细胞中的作用 ,以期丰富Bcl 2及其反义核酸调控肿瘤细胞凋亡的资料。方法 将反义Bcl 2基因转染于U937细胞 ,建立U937/as bcl2细胞模型 ,经流式细胞技术检测模型细胞Bcl 2的的表达水平 ,通过细胞计数观察模型细胞在正常培养条件下和有重组人肿瘤坏死因子α (rhTNFα)存在时的生长和存活特性变化。结果 ①U937/as bcl2模型细胞的Bcl 2蛋白表达水平明显下降 ,但其在正常培养条件下的生长和存活能力无明显变化。②在rhTNFα的作用下 ,模型细胞的存活率较对照细胞者明显下降。结论 ①单独反义bcl 2基因转染可明显抑制U937细胞Bcl 2的表达 ,但对细胞在正常培养条件下的生长和存活能力无明显影响。②反义Bcl 2基因转染可增加U937细胞对TNFα的敏感性
Objective To investigate the role of Bcl-2 antisense gene in monocytic leukemia cell line U937 in order to enrich the information of Bcl-2 and its antisense nucleic acid in regulating tumor cell apoptosis. Methods The antisense Bcl 2 gene was transfected into U937 cells and the U937 / as bcl2 cell model was established. The expression of Bcl 2 was detected by flow cytometry. The cells were observed under the normal culture conditions Changes in growth and survival characteristics of human tumor necrosis factor alpha (rhTNFα). Results ① The expression of Bcl 2 protein in U937 / as bcl2 cells was significantly decreased, but the growth and viability of U937 / as bcl2 cells did not change significantly under normal culture conditions. ② Under the action of rhTNFα, the survival rate of model cells was significantly lower than that of control cells. Conclusion ① The transfection of antisense bcl 2 gene can significantly inhibit the expression of Bcl 2 in U937 cells, but has no significant effect on the growth and viability of cells under normal culture conditions. ② Antisense Bcl 2 gene transfection can increase the sensitivity of U937 cells to TNFα