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目的研究大鼠脑缺血／再灌注时热休克蛋白（ＨＳＰ）７０表达及川芎嗪对ＨＳＰ７０表达的影响。方法采用ＳＤ大鼠左侧颈总动脉结扎并滴注生理盐水脑缺血／再灌注模型，以免疫细胞化学法检测２４只缺血／再灌注及川芎嗪干预大鼠脑缺血／再灌注３０ｍｉｎ时脑组织ＨＳＰ７０表达并与病理组织学改变进行对照研究。结果（１）非缺血侧脑组织无ＨＳＰ７０表达，缺血３０ｍｉｎ缺血侧大脑皮层可见ＨＳＰ７０表达；再灌注３０ｍｉｎ组仅１只大鼠（１／６）缺血侧大脑皮层有ＨＳＰ７０表达。（２）与单纯缺血大鼠相比，川芎嗪干预组缺血侧大脑皮层ＨＳＰ７０表达明显增强，计算机辅助图像半定量分析ＨＳＰ７０免疫阳性细胞数，分别为平均４３．５５±１２．５１个／片和８４．９５±１６．７个／片（Ｐ＜０．０１）。（３）缺血侧皮层神经细胞呈现轻度缺血改变，川芎嗪干预大鼠缺血损伤程度似有减轻。结论脑缺血时可诱导缺血脑细胞部分ＨＳＰ７０表达，川芎嗪干预可促使缺血大脑皮层ＨＳＰ７０表达明显增强，皮层神经细胞的缺血损伤有所减轻。
Objective To investigate the expression of heat shock protein 70 (HSP70) and the effect of ligustrazine on the expression of heat shock protein 70 (HSP70) during cerebral ischemia / reperfusion in rats. Methods Twenty-four ischemia / reperfusion and ligustrazine-induced cerebral ischemia / reperfusion in rats were performed by ligation of the common carotid arteries of the left middle cerebral artery of SD rats and the model of normal saline cerebral ischemia / reperfusion. When the expression of HSP70 in brain tissue and pathological changes were compared. Results (1) HSP70 expression was found in non-ischemic brain tissue, and expression of HSP70 was observed in ischemic cortex 30 min after ischemia. Only 1 rat in 30 min reperfusion group (1/6) had HSP70 expression in ischemic cortex. (2) Compared with the ischemia-reperfusion group, the expression of HSP70 in the ischemic cortex increased significantly in the Ligustrazine-treated group, and the number of HSP70 immunoreactive cells was semi-quantitative by computer-aided imaging (average 43.55 ± 12.51 / Tablets and 84.95 ± 16.7 tablets / tablet (P <0.01). (3) The ischemic cortex nerve cells showed mild ischemic changes, ligustrazine can reduce the severity of ischemic injury in rats. Conclusions Cerebral ischemia can induce partial expression of HSP70 in ischemic brain cells. Intervention with ligustrazine can significantly increase the expression of HSP70 in ischemic cortex and reduce the ischemic injury of cortical neurons.