论文部分内容阅读
1986年5月,我们从啥尔滨市第一医院一例住院病人——肺结核合并大叶性肺炎的病人痰中,检出一株军团病杆菌,经鉴定为嗜肺性军团病杆菌第Ⅰ型,菌株编号为Hrb861。所分离的Hrb861株菌,为革兰氏阴性杆菌,在含硝酸铁军团菌选择性琼脂培养基上生长良好,而在其它营养琼脂上不生长。不发酵糖类,氧化酶弱阳性,接触酶阳性,产生淀粉水解酶,硝酸盐还原阴性,动力阳性,符合军团病杆菌特征。直接单克隆荧光抗体染色和试管定量凝集试验,证实Hrb861株为嗜肺性军团病杆菌血清
In May 1986, we detected a Legionella pneumophila from the sputum of a patient with pulmonary tuberculosis complicated with lobar pneumonia, a case of Harbin First City Hospital. It was identified as Legionella pneumophila type I , Strain number Hrb861. The isolated Hrb861 strain, a Gram-negative bacterium, grew well on selective agar medium containing Legionella fernatis and did not grow on other nutrient agar. Non-fermenting carbohydrates, weakly positive for oxidase, positive for contacting enzyme, produce starch hydrolyzing enzyme, negative for nitrate reduction, positive for power, in line with the characteristics of Legionellae. Direct monoclonal antibody staining and tube quantitative agglutination test confirmed that Hrb861 strain is Legionella pneumophila bacilli serum