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目的探讨用碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)及其不同浓度单克隆抗体(bFGF-Mab)的方法对裸鼠人侵袭性垂体腺瘤移植瘤血管生成和肿瘤生长的影响及可能机制。方法建立50只人侵袭性垂体腺瘤裸鼠皮下移植瘤模型,按区组随机排列方法分成bFGF组,30、20、10μg/ml 3个抗体浓度的bFGF-Mab组及生理盐水对照组,每组10只,分别给予不同处理后8周终止实验,每周测量肿瘤体积,绘制移植瘤体积曲线;对移植瘤组织用免疫组化法检测CD31的表达,以计算肿瘤组织微血管密度(microvessel density,MVD)。结果与对照组、bFGF组比较,bFGF-Mab组对移植瘤的生长及肿瘤血管生成有明显抑制作用,以30μg/ml组的抑瘤率(55.71%)最高(P<0.01);bFGF-Mab各浓度组肿瘤组织中MVD均明显下降,30μg/ml组(3.36±1.26)下降最明显(P<0.01)。结论 bFGF-Mab处理后对裸鼠侵袭性垂体腺瘤皮下移植瘤具有较强的体内抑瘤效应,可能是通过抑制细胞增殖活性及肿瘤血管生成而达到抑制垂体腺瘤的侵袭性生长的。
Objective To investigate the effects of basic fibroblast growth factor (bFGF) and different concentrations of monoclonal antibody (bFGF-Mab) on angiogenesis and tumor growth in human invasive pituitary adenoma And possible mechanism. Methods Fifty human invasive pituitary adenomas subcutaneously transplanted in nude mice were established and randomly divided into bFGF group, bFGF-Mab group at 30, 20, 10μg / ml antibody concentration and normal saline control group (N = 10). The mice were sacrificed at 8 weeks after treatment. The volume of tumor was measured weekly and the volume of tumor was plotted. The expression of CD31 was detected by immunohistochemistry in order to calculate the microvessel density MVD). Results Compared with the control group and bFGF group, the bFGF-Mab group had a significant inhibitory effect on the growth and tumor angiogenesis of the xenografted tumor. The inhibition rate of the bFGF-Mab group was the highest (55.71%) at 30μg / ml (p <0.01) MVD in each group decreased significantly (P <0.01), but decreased significantly in 30μg / ml group (3.36 ± 1.26) (P <0.01). Conclusion The bFGF-Mab treatment has a strong in vivo antitumor effect on the subcutaneous xenografts of invasive pituitary adenoma in nude mice, which may inhibit the invasive growth of pituitary adenoma by inhibiting cell proliferation activity and tumor angiogenesis.