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本文采用引物Ｒ２２２和Ｒ３３３，对来自我国新疆克拉玛依地区的１２份皮肤利什曼病（ＣＬ）患者的皮肤病变组织标本进行ＰＣＲ扩增。结果显示１２例ＣＬ患者病变组织均出现预期的３９７ｂｐ的阳性扩增区带。同时用地高辛标记的Ｌ．ｔｒｏｐｉｃａＳＳＵｒＤＮＡ扩增产物探针进行斑点杂交及Ｓｏｕｔｈｅｒｎ印迹杂交。斑点杂交结果显示：Ｌ．ｔｒｏｐｉｃａＳＳＵｒＤＮＡ扩增产物探针只与１２例ＣＬ患者的ＣＬ－ＰＣＲ－ＡＰ出现阳性杂交信号，与Ｌ．ｔｕｒａｎｉｃａ、Ｌ．ｉｎｆａｎｔｕｍ无阳性杂交信号，上述探针与１２例ＣＬ患者的ＣＬ－ＰＣＲ－ＡＰＳｏｕｔｈｅｒｎ印迹杂交均出现阳性杂交区带。以上结果证实Ｌ．ｔｒｏｐｉｃａ与我国新疆克拉玛依地区皮肤利氏曼病病原体ＳＳＵｒＤＮＡＰＣＲ扩增产物存在同源关系
In this study, we used primers R222 and R333 to amplify skin lesions of 12 skin leishmaniasis (CL) patients from Karamay, Xinjiang. The results showed that 12 cases of CL patients showed the expected 397bp positive amplification zone lesions. Also labeled with digoxin. tropicaSSUrDNA amplification product probe for dot hybridization and Southern blot hybridization. Dot blot results show: L. The probe of tropicaSSUrDNA amplification product only showed positive hybridization signal with CL-PCR-AP of 12 patients with CL, turanica, L. infantum no positive hybridization signal, the above probe and 12 cases of CL patients with CL-PCR-APSouthern blot hybridization showed positive hybridization zone. The above results confirm L. There is a homology relationship between tropica and SSUrDNAPCR amplification products of dermatomyositis Rickettsia pathogens in Karamay, Xinjiang